Universal Primers
|
# |
Primer |
Sequences (5' ~ 3') |
Plasmid |
|
1 |
28 glll |
GTATGGGATTTTGCTAAACAAC |
Ph.D. glll |
|
1a |
-96 glll |
CCCTCATAGTTAGCGTAACG |
Ph.D. glll |
|
2 |
Ac5 Forward |
ACACAAAGCCGCTCCATCAG |
pAc5.1/V5-His |
|
3 |
AD Reverse |
AGATGGTGCACGATGCACAG |
AD |
|
4 |
a-Factor |
TACTATTGCCAGCATTGCTGC |
Pichia (pMET) |
|
5 |
AOX1 Forward |
GACTGGTTCCAATTGACAAGC |
Pichia |
|
6 |
AOX1 Reverse |
GCAAATGGCATTCTGACATCC |
Pichia |
|
7 |
AS HSV Tag Primer |
ATCCTCGGGGTCTTCCG |
pIEx |
|
8 |
AS SoTag 18mer Primer |
GTCCATGTGCTGGCGTTC |
pIEx |
|
9 |
AUG1 Forward |
CAATTTACATCTTTATTTATTAACG |
Pichia |
|
10 |
AUG1 Reverse |
GAAGAGAAAAACATTAGTTGGC |
Pichia |
|
11 |
Bac Forward |
TTTTACTGTTTTCGTAACAGTTTT |
pBlueBac4.5 |
|
12 |
Bac Reverse |
CGGATTTCCTTGAAGAGAGTA |
pBlueBacHis2 |
|
13 |
Baculovirus (+15)Reverse |
ACTTCAAGGAGAATTTCC |
pMelBac |
|
14 |
BGH Reverse |
TAGAAGGCACAGTCGAGG |
Universal |
|
15 |
BK Reverse |
ACAGGAAACAGCTATGACCTTG |
BK virus |
|
16 |
BKRSV |
CGCCATTTGACCATTCA |
pBK-rsv |
|
17 |
Bluescript KS |
TCGAGGTCGACGGTATC |
pBluescript |
|
18 |
Bluescript SK |
CGCTCTAGAACTAGTGGATC |
pBluescript |
|
19 |
CBDcenA |
TCAACGGCACCACCTGCA |
|
|
20 |
CBDcexLEAD |
TAGGTGCAACTGTTGTTCTG |
|
|
21 |
CBDclos |
CAACACCAGTTGTAAATCCA |
|
|
22 |
cI Forward |
GGATAGCGGTCAGGTGTT |
pHybcI/HK |
|
23 |
Cite primer |
GGGGACGTGGTTTTCCTTTG |
pCITE |
|
24 |
CMV Forward |
CGCAAATGGGCGGTAGGCGTG |
Universal |
|
25 |
CYC1 Reverse |
GCGTGAATGTAAGCGTGAC |
Cyc1 |
|
26 |
DsbA |
CGAGTATGCTGATACAGTGA |
DsRed |
|
27 |
DsbC |
GAATTTCTCGACGAACACCA |
DsRed |
|
28 |
DsRed1-C |
AGCTGGACATCACCTCCCACAACG |
DsRed |
|
29 |
DsRed1-N |
GTACTGGAACTGGGGGGACAG |
DsRed |
|
30 |
DsRed-C |
AAGAAGCCTGTGCAGCTACCAGG |
DsRed |
|
31 |
DsRed-N |
CGCCTTCTATTTCAAACTCGTGCC |
DsRed |
|
32 |
EBV Reverse |
GTGGTTTGTCCAAACTCATC |
EBV |
|
33 |
EF-1a Forward |
TCAAGCCTCAGACAGTGGTTC |
|
|
34 |
EGFP-C |
CATGGTCCTGCTGGAGTTCGTG |
EGFP |
|
35 |
EGFP-N |
CGTCGCCGTCCAGCTCGACCAG |
EGFP |
|
36 |
GAL1 Forward |
AATATACCTCTATACTTTAACGTC |
Gal1 |
|
37 |
Glprimer 1 |
TGTATCTTATGGTACTGTAACTG |
|
|
38 |
Glprimer 2 |
CTTTATGTTTTTGGCGTCTTCCA |
|
|
39 |
gp64 promoter |
CTACTAGTAAATCAGTCACACC |
|
|
40 |
gp64 Signal primer |
GCGCTATTGTTTTATATGTGC |
|
|
41 |
IE1 promoter primer |
TGGATATTGTTTCAGTTGCAAG |
pIEx, pBIEx |
|
42 |
Lamdagt10For |
CTTTTGAGCAAGTTCAGGCCTGGTTAAG |
Lambda GT10 |
|
43 |
Lamdagt10Rev |
GAGGTGGCTTATGAGTATTTCTTCCAGG |
Lambda GT10 |
|
44 |
Lamdagt11For |
GGTGGCGACGACTCCTGGAGCCCG |
Lambda GT11 |
|
45 |
Lamdagt11Rev |
TTGACACCAGACCAACTGGTAATG |
Lambda GT11 |
|
46 |
M13 Forward (-20) |
GTAAAACGACGGCCAG |
Universal |
|
47 |
M13 Forward (-40) |
GTTTTCCCAGTCACGAC |
Universal |
|
47b |
M13 Reverse |
CAGGAAACAGCTATGAC |
Universal |
|
48 |
pUCR/M13 Forward |
CCCAGTCACGACGTTGTAAAACG |
pUC |
|
49 |
pUCR/M13 Reverse |
AGCGGATAACAATTTCACACAGGAA |
pUC |
|
50 |
MT Forward |
CATCTCAGTGCAACTAAA |
pMT/V5-His |
|
51 |
myc-His Reverse |
ATGACCGGTATGCATATTCAG |
|
|
52 |
OpIE2 Forward |
CGCAACGATCTGGTAAACAC |
pMIB/V5-His |
|
53 |
OpIE2 Reverse |
GACAATACAAACTAAGATTTAGTCAG |
pMIB/V5-His |
|
54 |
p10 Forward |
GTATATTAATTAAAATACTATACTG |
pTriEx-2 Hygro |
|
55 |
pBAD Forward |
ATGCCATAGCATTTTTATCC |
E.coli araBAD |
|
56 |
pBAD Reverse |
GATTTAATCTGTATCAGG |
E.coli araBAD |
|
57 |
pCDM8 Reverse |
TAAGGTTCCTTCACAAAG |
pCDM8 |
|
58 |
pCEP Forward |
AGAGCTCGTTTAGTGAACCG |
|
|
59 |
pCMV Forward |
GATCCGGTACTAGAGGAACTGAAAAAC |
|
|
60 |
pDAB Forward |
ATGCCATAGCATTTTTATCC |
|
|
61 |
pET Upstream Primer |
ATGCGTCCGGCGTAGA (16) |
|
|
62 |
pETBlueDOWN Primer |
GTTAAATTGCTAACGCAGTCA |
|
|
63 |
pETBlueUP Primer |
TCACGACGTTGTAAAACGAC |
|
|
64 |
pFastBac Forward |
GGATTATTCATACCGTCCCA |
pFastBac |
|
65 |
pFastBac Reverse |
CAAATGTGGTATGGCTGATT |
pFastBac |
|
66 |
pGAP Forward |
GTCCCTATTTCAATCAATTGAA |
pGAPz |
|
67 |
pGENE Forward |
CTGCTATTCTGCTCAACCT |
|
|
68 |
pGEX 3' |
GAGCTGCATGTGTCAGAGG |
Universal |
|
69 |
pGEX 5' |
GGCAAGCCACGTTTGGTG |
Universal |
|
70 |
pHook Forward |
ACGGTGCATTGGAACGGAC |
pHook-2, -3 |
|
71 |
pHook Reverse |
GATTGCGTCGCATCGACCC |
pHook-2, -3 |
|
72 |
pHybLex/Zeo Forward |
AGGGCTGGCGGTTGGGGTTATTCGC |
pHybLex/Zeo |
|
73 |
pHybLex/Zeo Reverse |
GAGTCACTTTAAAATTTGTATACAC |
pHybLex/Zeo |
|
74 |
PinPoint Sequencing primer |
CGTGACGCGGTGGAGGGCG |
PinPoint Xa-1, -2, -3, PinPoint Xa-1 T-vector |
|
75 |
pJET1.2 Forward |
CGACTCACTATAGGGAGAGCGGC |
|
|
76 |
pJET1.2 Reverse |
AAGAACATCGATTTTCCATGGCAG |
|
|
77 |
pJG4-5 Forward |
GATGCCTCCTACCCTTATGATGTGCC |
pJG4-5 |
|
78 |
pJG4-5 Reverse |
GGAGACTTGACCAAACCTCTGGCG |
pJG4-5 |
|
79 |
Polyhedrin Forward |
AAATGATAACCATCTCGC |
pVL1393 |
|
80 |
Polyhedrin Reverse |
GTCCAAGTTTCCCTG (15) |
pVL1393 |
|
81 |
pQE-TriSystem Forward |
GTTATTGTGCTGTCTCATC |
|
|
82 |
pQE-TriSystem Reverse |
TCGATCTCAGTGGTATTTGTG |
|
|
83 |
pREP Forward |
GCTCGATACAATAAAGCCC |
pREP4 |
|
84 |
pRset |
CTAGTTATTGCTCAGCGGTGG |
pRset |
|
85 |
pRH Forward |
CTGTCTCTATACTCCCCTATAG |
pRH |
|
86 |
pRH Reverse |
CAAAATTCAATAGTTACTATCGC |
pRH |
|
87 |
pRSET Reverse |
TAGTTATTGCTCAGCGGTGG |
pRSET |
|
88 |
pTarget Sequencing Primer |
TTACGCCAAGTTATTTAGGTGACA |
pTarget |
|
89 |
pTrcHis Forward |
GAGGTATATATTAATGTATCG |
pTrcHis |
|
90 |
pTrcHis Reverse |
GATTTAATCTGTATCAGG |
pTrcHis |
|
91 |
pTRE 3' |
CCACACCTCCCCCTGAAC |
pTRE |
|
92 |
pTRE 5' |
CGCCTGGAGACGCCATCC |
pTRE |
|
93 |
pTriplEx 3' |
ACTCACTATAGGGCGAATTG |
pTriplEx |
|
94 |
pTriplEx 5' |
CTCGGGAAGCGCGCCATTGTGTTGGT |
pTriplEx |
|
95 |
pUni Forward |
CTATCAACAGGTTGAACTG |
pUni |
|
96 |
pUni Reverse |
CAGTCGAGGCTGATAGCGAGCT |
pUni |
|
97 |
pYESTrp Forward |
GATGTTAACGATACCAGCC |
pYESTrp |
|
98 |
pYESTrp Reverse |
GCGTGAATGTAAGCGTGAC |
pYESTrp |
|
99 |
QE Promoter |
CCGAAAAGTGCCACCTG |
pQE |
|
100 |
QE Reverse |
GTTCTGAGGTCATTACTGG |
pQE |
|
101 |
R-20mer Primer |
CAGCTATGACCATGATTACG |
pSTBlue-1 |
|
102 |
RsaA Reverse |
GCCGCGCCAGCGACGCGGAGGG |
pCX |
|
103 |
RVprimer3 |
CTAGCAAAATAGGCTGTCCC |
pGL / pCAT3 |
|
104 |
Rvprimer4 |
GACGATAGTCATGCCCCGCG |
pGL / pCAT3 |
|
105 |
SeqL-A (proximal to attL1) |
TCGCGTTAACGCTAGCATGGATCTC |
pDONR201/pDONR207 |
|
106 |
SeqL-B (proximal to attL2) |
GTAACATCAGAGATTTTGAGACAC |
pDONR201/pDONR207 |
|
107 |
STag 18mer Primer |
GAACGCCAGCACATGGAC |
pIEx-1 |
|
108 |
STag Primer |
CGAACGCCAGCACATGGACA |
pIEx-1 |
|
109 |
Sp6 Promoter |
GATTTAGGTGACACTATAG |
Universal |
|
110 |
SV40-pArev |
CCTCTACAAATGTGGTATGG |
pRL-SV40 |
|
111 |
SV40-Promoter |
GCCCCTAACTCCGCCCATCC |
pRL-SV40 |
|
112 |
T3 Promoter |
ATTAACCCTCACTAAAGGGA |
Universal |
|
113 |
T7 EEV |
ATGTCGTAATAACCCCGCCCCG |
pAlterMAX, pSI, pCI, pCI-Neo, pCMVTnT, pTnT, phMGFP Vector, HaloTag pHT2, psiCHECK-1, -2 |
|
114 |
T7 gene 10 Primer |
TGAGGTTGTAGAAGTTCCG |
|
|
115 |
T7 Promoter |
TAATACGACTCACTATAGGG |
Universal |
|
116 |
T7 Reverse |
TAGTTATTGCTCAGCGGTGG |
Universal |
|
117 |
T7 Terminator |
GCTAGTTATTGCTCAGCGG |
Universal |
|
118 |
U6 Primer |
GGGCAGGAAGAGGGCCTAT |
|
|
119 |
U-19mer Primer |
GTTTTCCCAGTCACGACGT |
M13mp18, pCITE |
|
121 |
V5 Reverse Primer |
ACCGAGGAGAGGGTTAGGGAT |
V5 Epitope |
|
122 |
VP22 Forward |
GGCCACGGCGACTCGA |
|
|
123 |
Xpress Forward |
TATGGCTAGCATGACTGGT |
Xpress Epitope |
|
124 |
VLH |
TTGTGTGGAATTGTGAGCGG |
|
|
125 |
VF2 |
TGCCACCTGACGTCTAAGAA |
|
|
126 |
VR |
ATTACCGCCTTTGAGTGAGC |
|
|
127 |
TKpA Rev |
CTTCCGTGTTTCAGTTAGC |
|
|
128 |
pQE60-FW |
CCCGAAAAGTGCCACCTG |
|
|
129 |
pQE60-Rv |
GTTCTGAGGTCATTACTGG |
|
|
130 |
SeqL-A (ATTL1) |
GCGAGAGTAGGGAACTGC |
pENTR |
|
131 |
SeqL-B (ATTL2) |
AACATCAGAGATTTTGAGACAC |
pENTR |
|
132 |
pmirGlo F (7205bp) |
GAGGTGCCTAAAGGACTGAC |
pmirGlo |